csf3  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Advanced Science

Article Title: Reprogramming of Fatty Acid Metabolism via PPARα‐Orchestrated FADS2 in Keratinocytes Modulates Skin Inflammation in Psoriasis

doi: 10.1002/advs.202417049

Figure Lengend Snippet: PPARα acts as an upstream positive regulator of FADS2 in psoriatic keratinocytes. A) RT‐qPCR analysis of PPARA expression in normal skin from healthy controls (NN, n = 4) and lesional skin tissue from psoriasis patients (PS, n = 5). B) Representative immunofluorescence images of PPARα and K14 co‐staining in normal skin from healthy controls and lesional skin tissue from psoriasis patients. C) RT‐qPCR analysis of PPARA in psoriatic lesional skin before and 10 weeks after infliximab treatment (n = 5). D) Representative images of PPARα immunofluorescence staining in lesional skin from psoriasis patients at baseline and week 10 following infliximab treatment. E) Representative immunofluorescence images of PPARα staining in healthy skin from control mice and skin lesions from IMQ‐induced psoriasis mouse model. F,G) RT‐qPCR analysis of PPARA (F) and FADS2 (G) expression in HaCaT cells stimulated with M5 for 12 h (n = 3). H) Immunoblotting of PPARα and FADS2 in HaCaT cells stimulated with M5 cytokines for the indicated time. I) RT‐qPCR analysis of PPARA , FADS2 , and indicated genes in HaCaT cells transfected with PPARA siRNA (si PPARA ) and control siRNA (siNC) for 24 h, followed by PBS or M5 stimulation for 12 h (n = 3). J) RT‐qPCR analysis of FADS2 and indicated genes in HaCaT cells pretreated with WY14643 or DMSO for 21 h, followed by PBS or M5 stimulation for 3 h (n = 3). K) ELISA quantification of CXCL1, CXCL8, and CSF3 protein levels in cell lysates and supernatants from HaCaT cells treated as in (J) (n = 3). L) RT‐qPCR analysis of the indicated genes in HaCaT cells transfected with si FADS2 and siNC for 24 h, followed by M5 stimulation for 3 h before WY14643 or DMSO pretreatment (n = 3). Scale bar, 50 µm. Data are presented as mean ± SD. Statistical significance was determined by unpaired two‐tailed Student's t test (A,F,G,K), paired two‐tailed Student's t test (C), or one‐way ANOVA (I,J,L). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.

Article Snippet: Similarly, human CXCL1 (#70‐EK196), CSF3 (#70‐EK169), and CXCL8 (#70‐EK108/2) levels in cultured human keratinocyte lysates and supernatants were measured using corresponding human ELISA kits (Lianke Bio, China) according to the provided protocols.

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Control, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: The FASEB Journal

Article Title: A Neutrophil Storage Pool in the Fetal Liver is the Principal Source of the Postnatal Neutrophil Surge

doi: 10.1096/fj.202501613RR

Figure Lengend Snippet: Neutrophil accumulation was mediated by G‐CSF in the mouse fetal liver. (A) Experimental scheme of BrdU treatment and flow cytometry for fetal liver. (B) Representative flow cytometry plots for fetal livers collected from vehicle‐injected dam or BrdU‐injected dam. (C) Representative flow cytometry plots for CMP, MEP, and GMP in the fetal livers collected from BrdU‐injected pregnant mice at e14, e16, or e18. (D) Changes in the cell counts and (E) the BrdU‐positive rate in (C) with e12 data added. (B–E) The fetal livers were collected after 90 min of BrdU injection. n = 8–13 from two independent dams for each group. $$ p < 0.01; $$$ p < 0.001 (vs. e12), ## p < 0.01; ### p < 0.001 (vs. e14) and * p < 0.05; ** p < 0.01; *** p < 0.001 (vs. e16) by one‐way ANOVA followed by Tukey's multiple comparison tests. ns, not significant. (F) Representative flow cytometry plots of fetal livers at e15 or e17. (G) Changes in the cell counts and (H) representative histograms and the BrdU‐positive rates in (F). (F–H) The fetal livers were collected after 6 h of BrdU injection. n = 8–9 from two independent dams for each group. *** p < 0.001 vs. e15 by two‐way ANOVA followed by Sidak's multiple comparisons test. (I) Experimental scheme of G‐CSF neutralizing antibody treatment and the representative flow cytometry plots of the fetal livers at e17. (J) Cell counts of neutrophils and monocyte lineage in (J). n = 14 from three independent dams for each group. ** p < 0.01 by unpaired Student's t ‐test. ns, not significant. (K) Relative mRNA expression of Csf3 (G‐CSF) gene in the placenta, umbilical artery, and umbilical vein from rat fetuses on e19 or e21. n = 6 from two individual dams for each group. * p < 0.05; *** p < 0.001 by two‐way ANOVA followed by Sidak's multiple comparisons test.

Article Snippet: Expression of rat Csf3 mRNA ( NM_017104.2 ) was evaluated by the TaqMan Gene Expression Assay (Rn00567344_m1, ThermoFisher).

Techniques: Flow Cytometry, Injection, Comparison, Expressing